Journal: Memórias do Instituto Oswaldo Cruz
Article Title: Concomitant immunity against superimposed homologous Echinostoma caproni infections in mice is mediated by interleuquin-25
doi: 10.1590/0074-02760250004
Figure Lengend Snippet: primary and superimposed infection induce different patterns of macrophage activation. (A) Indirect immunofluorescence with recombinant anti-CD16-2/FCGR4/Fc for M1 and antibody MR/CD206/Mannose Receptor for M2 (red) on intestinal tissue of Institute of Cancer Research (ICR) naïve and mice after Echinostoma caproni primary, superimposed infection in mice and superimposed infections in anti-interleuquin-25 (α-IL-25)-treated mice (scale bar: 100 mm). (B) Quantitation of the percentage of area covered by anti-CD16-2/FCGR4/Fc for M1 and antibody MR/CD206/Mannose Receptor staining. Vertical bars show standard deviation. a: significant differences with respect to negative controls; b: significant differences with respect to primary infections; c: significant differences with respect to superimposed infection- in α-IL-25-treated mice (p < 0.05).
Article Snippet: A rabbit anti-CD16-2/FCGR4/Fc antibody (Sino Biological, clone no. 012), and a goat antibody MR/CD206/Mannose Receptor (Novus Invitro Technologies Ltd, Colorado, USA) were used for labelling M1 and M2, respectively.
Techniques: Infection, Activation Assay, Immunofluorescence, Recombinant, Quantitation Assay, Staining, Standard Deviation